abstract：:We described 27 polyclonal colistin-resistant Enterobacteriaceae (MIC 4-16 μg/mL) infections (12 pneumonia, 12 urinary tract infection (UTI), two Bacteremia, and one skin/soft tissue infection) in which 74% harbored KPC. The isolates were polyclonal, 6 STs were identified and the colistin resistance was due to chromos...

journal_title：Diagnostic microbiology and infectious disease

authors： de Maio Carrillho CM,Gaudereto JJ,Martins RC,de Castro Lima VA,de Oliveira LM,Urbano MR,Perozin JS,Levin AS,Costa SF

更新日期：2017-03-01 00:00:00

abstract：:The broth microdilution (BMD) MIC testing method for telavancin was recently revised BMD (rBMD) to improve accuracy and reproducibility. Staphylococcus aureus isolates from telavancin phase 3 complicated skin and skin-structure infection (cSSSI) studies were tested using the rBMD method. Retesting of 1132 isolates pro...

journal_title：Diagnostic microbiology and infectious disease

authors： Smart JI,Corey GR,Stryjewski ME,Wang W,Barriere SL

更新日期：2017-03-01 00:00:00

abstract：:In this study we evaluated the performance of the SHIGA TOXIN QUIK CHEK (Techlab®, Blacksburg, VA) and the ImmunoCard STAT! Enterohaemorrhagic E. coli (EHEC) (Meridian BioScience, Cincinnati, OH, USA) assays as methods for qualitatively detecting the presence of Shiga toxin in human fecal specimens. A multiplex PCR fo...

journal_title：Diagnostic microbiology and infectious disease

authors： Staples M,Fang NX,Graham RM,Smith HV,Jennison AV

更新日期：2017-02-01 00:00:00

abstract：:The species distribution and drug susceptibilities of 1106 Candida isolates collected in Taiwan Surveillance of Antimicrobial Resistance of Yeasts (TSARY) in 2014 were determined. Candida albicans is still the dominant species, accounting for 35.9%, followed by 28.3% C. glabrata, 26.6% C. tropicalis, 5.2% C. parapsilo...

journal_title：Diagnostic microbiology and infectious disease

authors： Zhou ZL,Lin CC,Chu WL,Yang YL,Lo HJ,TSARY Hospitals.

更新日期：2016-12-01 00:00:00

abstract：:Vitek® 2 (bioMérieux) is a widely used commercial antimicrobial susceptibility test (AST) system. AST-N244 card includes cephalothin as first-generation cephalosporin. We compared the cephalothin susceptibility results obtained with Vitek® 2 AST-N244 to those obtained by broth microdilution (BMD) and disk diffusion (D...

journal_title：Diagnostic microbiology and infectious disease

authors： López IA,Montes JC,Álvarez MJ,Mazarrasa CF,Martínez-Martínez L

更新日期：2016-12-01 00:00:00

abstract：:Data about the performance of MALDI-TOF mass spectrometry against cryptic Candida species are limited. According to our findings within the C. parapsilosis species complex, microbiologists should be aware that the choice of the instrument is critical for accurate species identification due to the risk of misidentifica...

journal_title：Diagnostic microbiology and infectious disease

authors： Haas M,Grenouillet F,Loubersac S,Ariza B,Pepin-Puget L,Alvarez-Moreno CA,Valderrama-Beltrán SL,Lavergne RA,Le Pape P,Morio F

更新日期：2016-12-01 00:00:00

abstract：:The aims of this prospective study were to examine the correlation between the molecular types and the antimicrobial susceptibility patterns of Clostridium difficile isolates with the source of acquisition and the occurrence of C. difficile infections (CDI) in a tertiary center in Israel. All available isolates from c...

journal_title：Diagnostic microbiology and infectious disease

authors： Miller-Roll T,Na'amnih W,Cohen D,Carmeli Y,Adler A

更新日期：2016-12-01 00:00:00

abstract：:Mycobacterial identification using MALDI-ToF MS (MALDI) has been hindered by inadequate extraction methods. Adaptive Focused Acoustics™ uses concentrated ultrasonic energy to achieve cellular disruption. Using this technology, we developed a rapid mycobacterial inactivation/protein extraction method for MALDI-based id...

journal_title：Diagnostic microbiology and infectious disease

authors： Adams LL,Dionne K,Fisher S,Parrish N

更新日期：2016-11-01 00:00:00

abstract：:Here we describe the relationships between serotypes, genotypes, and antimicrobial susceptibility among isolates causing invasive pneumococcal disease in Alaskan children during the pneumococcal conjugate vaccine (PCV) era. From 2001 to 2013 we received 271 isolates representing 33 serotypes. The most common serotypes...

journal_title：Diagnostic microbiology and infectious disease

authors： Miernyk KM,Bulkow LR,Case SL,Zulz T,Bruce MG,Harker-Jones M,Hurlburt DA,Hennessy TW,Rudolph KM

更新日期：2016-10-01 00:00:00

abstract：:Increased use of colistin in a clinical setting had resulted in the emergence of colistin-resistant (CoR) Acinetobacter baumannii. Combination therapy has been studied as a new approach to treat infections caused by A. baumannii. Here, we investigated the in vitro antimicrobial synergistic activities of several antimi...

journal_title：Diagnostic microbiology and infectious disease

authors： Hong DJ,Kim JO,Lee H,Yoon EJ,Jeong SH,Yong D,Lee K

更新日期：2016-10-01 00:00:00

abstract：BACKGROUND:Pneumococcal parapneumonic effusion seems to be increasing in children in the postvaccine era and is frequently associated with negative culture. Due to the low yield of culture, culture-independent tools are evaluated. METHODS:Culture-negative pleural fluid specimens from 38 children with parapneumonic eff...

journal_title：Diagnostic microbiology and infectious disease

authors： Perez VP,Caierão J,Fischer GB,Dias CA,d'Azevedo PA

更新日期：2016-10-01 00:00:00

abstract：BACKGROUND AND AIM:Helicobacter pylori can survive long incubation periods under anaerobic conditions, and should be possible to isolate under anaerobic conditions. Our aim was to isolate H. pylori in anaerobic conditions, from gastric biopsies of H. pylori infected patients. METHODS:We enrolled 27 patients with bleed...

journal_title：Diagnostic microbiology and infectious disease

authors： Perez-Perez GI,Van TN,Thu Huong D,Zhan G,Nguyet Anh D,Nguyet NT,Thi LT,Van Thinh N,Hong-Hanh NT

更新日期：2016-10-01 00:00:00

abstract：:Since the introduction of matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) in routine microbiology laboratories, identification of anaerobic bacteria has become easier. These increased possibilities provide new challenges concerning analytical workup and reporting of anaerobe...

journal_title：Diagnostic microbiology and infectious disease

authors： Peeters B,Magerman K,Waumans L,Cartuyvels R

更新日期：2016-09-01 00:00:00

abstract：:Phenotypic identification of coagulase-negative staphylococci (CoNS) is difficult and many staphylococcal species carry mecA. This study developed an array that was able to detect mecA and identify 30 staphylococcal species by targeting the internal transcribed spacer regions. A total of 129 target reference strains (...

journal_title：Diagnostic microbiology and infectious disease

authors： Han HW,Chang HC,Chang TC

更新日期：2016-09-01 00:00:00

abstract：:In this study, 185 nasopharyngeal swabs were tested to compare the sensitivity and specificity of the Luminex NxTAG (NxTAG) Respiratory Pathogen Panel (RPP) Assay with those of the Luminex Respiratory Virus Panel (RVP) Fast Assay v2 and singleplex real-time polymerase chain reaction (PCR). The NxTAG Assay identified a...

journal_title：Diagnostic microbiology and infectious disease

authors： Esposito S,Scala A,Bianchini S,Presicce ML,Mori A,Sciarrabba CS,Fior G,Principi N

更新日期：2016-09-01 00:00:00

abstract：:Molecular epidemiology allows us to know local HIV transmission and to design strategies of prevention. We studied 25 HIV newly diagnosed patients with the E138A mutation since the year 2010. Most transmission networks involved young and promiscuous men who have sex with men. Recent infection was only documented in pa...

journal_title：Diagnostic microbiology and infectious disease

authors： Chamizo F,Gilarranz R,Tosco T,Carrillo D,Holguín Á,Santana É,Pérez-Arellano JL,Hernández M,Francés A,Cárdenes MÁ,Zarzalejos JM,Pena-López MJ

更新日期：2016-09-01 00:00:00

abstract：:Nocardiosis occurs in both immunocompromised and immunocompetent patients. We aimed to assess how its characteristics differ depending on patients' immune status. Of a total of 54 patients with culture-proven nocardiosis diagnosed over 13 years, 18 (33%) were immunocompetent. Half of immunocompetent patients had chron...

journal_title：Diagnostic microbiology and infectious disease

authors： Kim YK,Sung H,Jung J,Yu SN,Lee JY,Kim SH,Choi SH,Kim YS,Woo JH,Lee SO,Chong YP

更新日期：2016-08-01 00:00:00

abstract：:Susceptibility testing using the EUCAST-AFST method against 39 clinical Fusarium strains consecutively collected from local and invasive infections during the last 10years assessed the in vitro activities of amphotericin B (AmB) and triazole antifungal agents. In addition, the susceptibility pattern of 12 reference st...

journal_title：Diagnostic microbiology and infectious disease

authors： Taj-Aldeen SJ,Salah H,Al-Hatmi AM,Hamed M,Theelen B,van Diepeningen AD,Boekhout T,Lass-Flörl C

更新日期：2016-08-01 00:00:00

abstract：:We evaluated the feasibility and efficacy of a commercially available methicillin-resistant Staphylococcus aureus (MRSA)-selective agar, chromID(™) MRSA, to detect group B streptococci with reduced penicillin susceptibility (PRGBS) in this study. The results showed 72.4% (21/29) sensitivity and 98.4% (60/61) specifici...

journal_title：Diagnostic microbiology and infectious disease

authors： Fukigai S,Morimoto M,Kimura K,Doyama Y,Miyazaki A,Kamiya C,Banno H,Morishima E,Onoda T,Nagano N,Jin W,Wachino JI,Yamada K,Arakawa Y

更新日期：2016-07-01 00:00:00

abstract：PURPOSE:In this retrospective study, we aimed to investigate the value of FDG-PET/CT in the diagnosis of spondylodiscitis (SD), the significance of dual time point imaging (DTPI) for SD diagnosis and the worth of SUVmax data for distinguishing tuberculous vs. non-tuberculous SD. MATERIALS AND METHODS:32 patients with ...

journal_title：Diagnostic microbiology and infectious disease

authors： Gunes BY,Onsel C,Sonmezoglu K,Ozaras R,Halac M,Tabak F,Sager S,Mete B,Sayman H,Kanmaz B

更新日期：2016-07-01 00:00:00

abstract：:Prophylactic antibiotics are an important measure in preventing perioperative infection, Failure to cover multidrug-resistant pathogens may place carriers at increased risk of infection. We conducted a prospective, cross-sectional study in patients prior to bowel surgery to measure the carriage prevalence of extended-...

journal_title：Diagnostic microbiology and infectious disease

authors： Pfeffer I,Zemel M,Kariv Y,Mishali H,Adler A,Braun T,Klein A,Matalon MK,Klausner J,Carmeli Y,Schwaber MJ

更新日期：2016-07-01 00:00:00

abstract：:Direct determination by pathogen-specific real-time PCR assay for Bacteroides fragilis was compared to culture in major abscess and diabetic foot infection biopsy samples. Real-time PCR resulted in an increased detection rate of 12% for B. fragilis and could improve the detection of B. fragilis in clinical samples. ...

journal_title：Diagnostic microbiology and infectious disease

authors： Stappers MH,Hagen F,Reimnitz P,Mouton JW,Meis JF,Gyssens IC

更新日期：2016-06-01 00:00:00

abstract：:Soft surfaces in the health-care setting harbor potentially pathogenic bacteria and fungi that can be transferred to patients and personnel. We evaluated the in vitro antimicrobial efficacy of two types of innovative copper-coated textiles against a variety of nosocomial multi-drug resistant (MDR) pathogens. Five isol...

journal_title：Diagnostic microbiology and infectious disease

authors： Irene G,Georgios P,Ioannis C,Anastasios T,Diamantis P,Marianthi C,Philippe W,Maria S

更新日期：2016-06-01 00:00:00

abstract：:We used 4 different bioinformatics algorithms to evaluate the application of a metagenomic shot-gun sequencing method in detection of Enterovirus D68 and other respiratory viruses in clinical specimens. Our data supported that next-generation sequencing, combined with improved bioinformatics tools, is practically feas...

journal_title：Diagnostic microbiology and infectious disease

authors： Huang W,Wang G,Lin H,Zhuge J,Nolan SM,Vail E,Dimitrova N,Fallon JT

更新日期：2016-05-01 00:00:00

abstract：:We conducted a series of polymerase chain reactions (PCRs) in order to detect bacteria (7 species) and viruses (17 species) in middle ear fluid (MEF) and nasopharynx (Nph) of children with acute otitis media (AOM; n=179). Bacterial and viral nucleic acids were detected in MEF of 78.8% and 14.5% patients, respectively....

journal_title：Diagnostic microbiology and infectious disease

authors： Yatsyshina S,Mayanskiy N,Shipulina O,Kulichenko T,Alyabieva N,Katosova L,Lazareva A,Skachkova T,Elkina M,Matosova S,Shipulin G

更新日期：2016-05-01 00:00:00

abstract：:We determined the MIC and minimum biofilm bactericidal concentration (MBBC) of tedizolid and vancomycin against 97 isolates of Staphylococcus aureus and 74 isolates of Staphylococcus epidermidis associated with prosthetic joint infection. All isolates were vancomycin susceptible in the planktonic state; all staphyloco...

journal_title：Diagnostic microbiology and infectious disease

authors： Schmidt-Malan SM,Greenwood Quaintance KE,Karau MJ,Patel R

更新日期：2016-05-01 00:00:00

abstract：:We report a case of mycotic keratitis caused by Bipolaris oryzae with predisposing trauma from a foreign body. The fungus was identified by sequencing the internal transcribed spacer region, translation elongation factor 1α (TEF1) gene, and partial glyceraldehyde-3-phosphate dehydrogenase (GPDH) gene, and the species ...

journal_title：Diagnostic microbiology and infectious disease

authors： Wang L,Al-Hatmi AM,Lai X,Peng L,Yang C,Lai H,Li J,Meis JF,de Hoog GS,Zhuo C,Chen M

更新日期：2016-05-01 00:00:00

abstract：:Sixty-three cases of Streptococcus pyogenes meningitis in adults were studied. Three predominant emm types were associated with meningitis: emm1 (44%), emm3 (11%), and emm6 (11%). Streptococcal toxic shock syndrome and mortality rates were 40% and 38%, respectively. ...

journal_title：Diagnostic microbiology and infectious disease

authors： Plainvert C,Doloy A,Joubrel C,Maataoui N,Dmytruk N,Touak G,Collobert G,Fouet A,Poyart C,Loubinoux J

更新日期：2016-04-01 00:00:00

abstract：:Comparison of methicillin-susceptible Staphylococcus aureus (MSSA) isolates responsible for bone and joint infection (BJI, n=73) and nasal colonization (n=57) revealed similar prevalence of β-lactamase (blaZ) type A production, associated with cefazolin hydrolysis, suggesting that blaZ type A-carrying MSSA isolates im...

journal_title：Diagnostic microbiology and infectious disease

authors： Trouillet-Assant S,Valour F,Mouton W,Martins-Simões P,Lustig S,Laurent F,Ferry T,Lyon BJI study group.

更新日期：2016-03-01 00:00:00

abstract：:Four immunocompromised patients, immigrants from Ethiopia, presented with diverse clinical manifestations of meningitis associated with Strongyloides stercoralis dissemination as determined by identification of intestinal larvae. The cerebrospinal fluid of 3 patients was tested by a validated (for stool) real-time PCR...

journal_title：Diagnostic microbiology and infectious disease

authors： Nadir E,Grossman T,Ciobotaro P,Attali M,Barkan D,Bardenstein R,Zimhony O

更新日期：2016-03-01 00:00:00

abstract：:We evaluate sequence data from the PathChip high-density hybridization array for epidemiological interpretation of detected pathogens. For influenza A, we derive similar relative outbreak clustering in phylogenetic trees from PathChip-derived compared to classical Sanger-derived sequences. For a positive polio detecti...

journal_title：Diagnostic microbiology and infectious disease

authors： Maurer-Stroh S,Lee CW,Patel C,Lucero M,Nohynek H,Sung WK,Murad C,Ma J,Hibberd ML,Wong CW,Simões EA

更新日期：2016-03-01 00:00:00

abstract：:A new real-time PCR assay for trichodysplasia spinulosa-associated polyomavirus (TSPyV) DNA detection was designed, and blood samples from kidney transplant recipients and healthy individuals were screened. TSPyV-DNA was not detected in blood from healthy individuals, but 26.8% of kidney recipients presented TSPyV-DNA...

journal_title：Diagnostic microbiology and infectious disease

authors： Urbano PR,Nali LH,Bicalho CS,Pierrotti LC,David-Neto E,Pannuti CS,Romano CM

更新日期：2016-02-01 00:00:00

abstract：:Data from recent conflicts related to war wounds and obligate anaerobes are limited. We define the epidemiology and antimicrobial susceptibility of obligate anaerobes from Iraq and Afghanistan casualties (6/2009-12/2013), as well as their association with clinical outcomes. Susceptibility against eleven antibiotics (7...

journal_title：Diagnostic microbiology and infectious disease

authors： White BK,Mende K,Weintrob AC,Beckius ML,Zera WC,Lu D,Bradley W,Tribble DR,Schnaubelt ER,Murray CK,Infectious Disease Clinical Research Program Trauma Infectious Disease Outcome Study Group.

更新日期：2016-02-01 00:00:00

abstract：:In 2011, a new mecA gene homolog, named mecC gene, was found in isolates from both humans and animals. The discovery of methicillin-resistant Staphylococcus aureus (MRSA) carrying the mecC gene has caused speculations about the origin, epidemiology, and impact of these isolates. The objective of this work is to perfor...

journal_title：Diagnostic microbiology and infectious disease

authors： Diaz R,Ramalheira E,Afreixo V,Gago B

更新日期：2016-02-01 00:00:00

abstract：:We evaluated the molecular epidemiology and microbiological characteristics of extended-spectrum β-lactamase-producing Escherichia coli (ESBL-EC) isolates that cause bacteremia in Korean hospitals, focusing especially on ST131. Our data suggest that ST131 isolates possessed more virulence traits and showed more multid...

journal_title：Diagnostic microbiology and infectious disease

authors： Cha MK,Kang CI,Kim SH,Cho SY,Ha YE,Wi YM,Chung DR,Peck KR,Song JH,Korean Network for Study on Infectious Diseases (KONSID).

更新日期：2016-02-01 00:00:00

abstract：:Few reports described the presence of bla(KPC) and qnr genes in the same isolate. This study reports the combination of bla(KPC-2) and qnrB19 genes in Klebsiella pneumoniae ST340 isolate in Brazil. These findings draw attention to this combination in ST340 isolate, which is part of the CC258, disseminated in Latin Ame...

journal_title：Diagnostic microbiology and infectious disease

authors： Martins WM,Almeida AC,Nicoletti AG,Cayô R,Gales AC,Alves LC,Brayner FB,Vilela MA,Morais MM

更新日期：2015-12-01 00:00:00

abstract：:The Verigene Gram-Negative Blood Culture Nucleic Acid Test (BC-GN) is a microarray-based assay that enables rapid detection of 9 common Gram-negative bacteria and 6 resistance determinants directly from positive blood cultures. We compared the performance of BC-GN with currently used automated systems, testing 141 cli...

journal_title：Diagnostic microbiology and infectious disease

authors： Uno N,Suzuki H,Yamakawa H,Yamada M,Yaguchi Y,Notake S,Tamai K,Yanagisawa H,Misawa S,Yanagihara K

更新日期：2015-12-01 00:00:00

abstract：:In a collection of 50 pvl-positive Staphylococcus aureus isolates from 10 Rio de Janeiro hospitals, 18 (36%) were from bloodstream infections, and 31 (62%) carried the SCCmec IV. Among 25 (50%) isolates of the USA1100/ST30/CC30 lineage present in 8 hospitals, 1 isolate was characterized as vancomycin-intermediate S. a...

journal_title：Diagnostic microbiology and infectious disease

authors： Chamon RC,Iorio NL,da Silva Ribeiro S,Cavalcante FS,Dos Santos KR

更新日期：2015-12-01 00:00:00

abstract：:Antibodies to phenolic glycolipid (PGL)-I and major membrane protein (MMP)-II were evaluated for serodiagnosis of leprosy in Southwest China, and the role in predicting the occurrence of the disease in household contacts (HHCs) of leprosy was examined. Using PGL-I (natural disaccharide-octyl-bovine serum albumin) anti...

journal_title：Diagnostic microbiology and infectious disease

authors： Wang H,Liu W,Jin Y,Yu M,Jiang H,Tamura T,Maeda Y,Makino M

更新日期：2015-11-01 00:00:00

abstract：:Timely and rapid diagnosis of multidrug resistance in tuberculous meningitis (TBM) is a challenge both for a microbiologist and neurologist. The present study was conducted to evaluate role of real-time polymerase chain reaction (PCR) using rpoB, IS6110, and MPB64 as targets in diagnosis of TBM in 110 patients and sub...

journal_title：Diagnostic microbiology and infectious disease

authors： Sharma K,Modi M,Kaur H,Sharma A,Ray P,Varma S

更新日期：2015-10-01 00:00:00